| 质粒名称: | pCLBW cox8 EGFP mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 78520 |
| 存储实验室: | David Chan |
|
载体骨架基本信息 |
|
| 载体名称: | pCLBW |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳细胞表达,逆转录病毒 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | 1492 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | cox8 EGFP mCherry（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XmaI（销毁后未知） |
| 3' 克隆位点: | XmaI（销毁后未知） |
| 5' 测序引物 | CMV |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCLBW cox8 EGFP mCherry was a gift from David Chan (Addgene plasmid # 78520 ; http://n2t.net/addgene:78520 ; RRID:Addgene_78520) |
| 参考文献部分: | Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1. Rojansky R, Cha MY, Chan DC. Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896. 10.7554/eLife.17896 PubMed 27852436 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCLBW cox8 EGFP mCherry 8923 bp ds-DNA circular SYN 06-JUL-2023
DEFINITION Fluorescent mitophagy reporter.
ACCESSION .
VERSION .
KEYWORDS pCLBW cox8 EGFP mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8923)
AUTHORS Rojansky R, Cha MY, Chan DC
TITLE Elimination of paternal mitochondria in mouse embryos occurs through
autophagic degradation dependent on PARKIN and MUL1.
JOURNAL Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
PUBMED 27852436
REFERENCE 2 (bases 1 to 8923)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 6, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8923
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 175..1191
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1115..1137
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1199..1218
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
protein_bind 1279..1303
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1310..1384
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 1406..2119
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 1406..2119
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(1448..1469)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1709..1728)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2056..2077
/label=EGFP-C
/note="EGFP, forward primer"
CDS 2135..2845
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2280..2298)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2548..2567
/label=mCherry-F
/note="mCherry, forward primer"
protein_bind complement(2846..2870)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 2915..3503
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2968..2988)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3386..3397)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3506..3522)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3507..3523)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 3840..4309
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4439..4456)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4610..5198)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4690..4709)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5358..6218)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5981..6000
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
enhancer 6370..6749
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 6750..6953
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 6904..6924
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 7192..7549
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 7614..8030
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 7960..7982
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 7998..8014
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 8040..8414
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 8352..8368
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
enhancer 8515..8818
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gcgggggggg ggggggggsg cgcgccgggg gggggggggg gggggggggg gggggggggg
61 gsgrggsggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
121 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
181 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
241 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
301 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
361 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
421 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
481 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
541 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
601 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
661 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
721 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
781 ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt
841 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
901 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
961 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1021 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
1081 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
1141 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
1201 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgatttg ataccgcggg
1261 cccgggatcc ctcgaatcac aagtttgtac aaaaaagcag gctgacacca tgtccgtcct
1321 gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc
1381 caagatccat tcgttggggg atccggtgag caagggcgag gagctgttca ccggggtggt
1441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
1501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
1561 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
1621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
1681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
1741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
1801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
1861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
1921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
1981 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
2041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
2101 catggacgag ctgtacaagg gctcaggctc gggcatggtg agcaagggcg aggaggataa
2161 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
2221 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
2281 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
2341 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
2401 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
2461 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
2521 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
2581 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
2641 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
2701 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
2761 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
2821 cggcatggac gagctgtata agtaaaccca gctttcttgt acaaagtggt gattcgaggc
2881 cgcgactcta gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
2941 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3001 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
3061 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
3121 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
3181 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
3241 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
3301 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
3361 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
3421 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
3481 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagga attccagctg
3541 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
3601 ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actattttcg agcggccgcc
3661 agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
3721 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
3781 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
3841 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
3901 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
3961 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
4021 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
4081 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
4141 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
4201 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
4261 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
4321 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
4381 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
5041 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
5281 caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag
5341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
5401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
5461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
5521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
5581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
5641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
5701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
5761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
5821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
5881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
5941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
6001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
6061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
6121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
6181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatgatcc
6241 ggccattagc catattattc attggttata tagcataaat caatattggc tattggccat
6301 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
6361 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
6421 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
6481 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
6541 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
6601 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
6661 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
6721 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
6781 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
6841 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
6901 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcaataaa
6961 agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac
7021 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
7081 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
7141 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
7201 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
7261 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
7321 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
7381 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg
7441 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
7501 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
7561 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga
7621 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
7681 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
7741 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
7801 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
7861 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
7921 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
7981 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc
8041 catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
8101 caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
8161 acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac
8221 ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag
8281 aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag
8341 acggcatcgc agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac
8401 catcctctag actgccggat cccagtgtgg tggtacggga attctgcagg aatctagtta
8461 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
8521 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
8581 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
8641 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
8701 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
8761 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
8821 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
8881 tttgtattta tttatttttt aattattttg tgcagcgatg ggg
//
